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Department of Neurobiology and Anatomy

MicroBrightfield's Neurolucida and Stereo Investigator

MicroBright

Neurolucida Summary
Neurolucida is advanced scientific software for performing brain mapping, neuron tracing, anatomical mapping, image analysis, and morphometry. Neurolucida can be used with live images from color video cameras or with stored image sets from confocal microscopes, electron microscopes, and scanning tomographic sources. When used on light microscopes, Neurolucida utilizes a computer controlled motorized XYZ stage for integrated navigation through tissue sections.

MicroBrightNeurolucida's neuron tracing capabilities include 3D measurement of bifurcating and trifurcating branching processes; measurement of process diameters; locations of spines, boutons, and other neuronal structures; reconstruction from neurons contained in single and serial sections; sophisticated morphometric analyses; and publication quality graphics output.

Neurolucida also features sophisticated tools for anatomical mapping. These mapping tools can be used to delineate anatomical regions, map locations of cell distributions, prepare anatomical maps for publication, and perform detailed morphometric analyses.

Serial sections of anatomical maps can be reconstructed in 3D. Each section can be aligned with the entire series to compensate for mounting irregularities. Shrinkage correction can be applied to compensate for differential tissue shrinkage between sections. 3D solid models can be created with transparency, perspective, and depth cuing.

A host of image analysis features include particle counting, interactive particle tracing, and densitometry.

Neurolucida uses many advanced computer-controlled microscopy techniques to obtain accurate results and speed your work. Neurolucida systems can be equipped with focus position encoders to obtain closed-loop focus control. Lens calibration includes automatic correction for Snell's law of optical refraction to allow accurate focal measurements using air, oil, and water immersion objectives. Features such as automatic parfocal and parcentric correction when switching objective lenses are invaluable and time saving.

Neurolucida data files are also compatible with a growing number of third party software products for electrotonic modeling and graphic display.

Stereo Investigator Summary
Stereo Investigator is advanced scientific software for performing design based stereology. Stereo Investigator can be used with live images from a color video camera, through the microscope oculars or with stored image sets from confocal microscopes, electron microscopes, and scanning tomographic sources. When used on light microscopes, Stereo Investigator utilizes a computer controlled motorized XYZ stage for integrated navigation through tissue sections and for performing highly efficient systematic random scans.

Since most stereological studies are used for analyzing anatomical material, Stereo Investigator also features sophisticated tools for anatomical mapping. These mapping tools can be used to delineate regions for performing stereological probes, to map locations of cell distributions, to prepare anatomical maps for publication, and to perform detailed morphometric analyses.

Stereo Investigator uses many advanced computer-controlled microscopy techniques to obtain accurate results and speed your work. Stereo Investigator systems can be equipped with focus position encoders to obtain closed-loop focus control. Lens calibration includes automatic correction for Snell's law of optical refraction to allow accurate focal measurements using air, oil, and water immersion objectives. Features such as automatic parfocal and parcentric correction when switching objective lenses are invaluable and time saving.

Stereo Investigator may be used to analyze Isotropic or Vertical (Anisotropic) sections. The estimation results and confidence levels (where applicable) of each analysis are calculated and displayed in spreadsheet format. These results can easily be transferred to other applications.